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Fish tissue extraktion and DNA isolation
Polymerase chain reaction (PCR)
Agarose gel electrophoresis
PCR product analysis- Genome database work and DNA marker screening
- Fish dissection and DNA isolation from different tissues
- Microscopic tissue analyses
- DNA purification and quality control - DNA concentration determination
- Gene-specific DNA primer design for DNA-based tests for species/sex differentiation
- Polymerase chain reaction (PCR)
- Agarose gel electrophoresis of PCR products
- PCR product purification, DNA sequence analysis
- Protocol, evaluation and discussion of results
